Among the different evolutionary forces driving population genetics, gene flow related to dispersal plays a major role in local adaptation processes. However, up to now very few studies on plant pathogenic fungi have attempted to decipher gene flow patterns at the scale of a production area. The causal agent of the black leaf streak disease on Banana crops, Mycosphaerella fijiensis, can be considered as a good biological model to study the adaptive potential of plant fungal pathogens: populations show relative demographic stability and panmixia so that most methods of population genetics can be properly applied. For gene flow analysis, we focussed on small farming production units which are scarcely distributed among large producing areas and rarely subjected to fungicide spraying. Previous population genetic studies of M. fijiensis allowed us to define the spatial scale to be considered and warned us about the effect of some putative barriers for gene flow analysis. Here we aimed at taking into account landscape in our population genetic analysis to i) delineate pathogen populations and determine the nature and importance of the barriers to gene flow and ii) assess M. fijiensis dispersal pattern within a continuous population. Around 850 isolates were sampled in a referenced area in Cameroon and genotyped using 22