The introduction in the late 90's of the world invasive B biotype of Bemisia tabaci into the island of La Réunion (South West of Indian Ocean) became a major problem both as a pest and as a vector of plant viruses. A preliminary genetic diversity study on B. tabaci revealed the presence of another biotype, indigenous of the South West of Indian Ocean: Ms. The genetic diversity of these two biotypes was extensively studied in la Réunion and the presence of a wild hybrid population between B and Ms biotypes was found. However, the proportion of hybrid individuals found was very low in the field; thus we addressed the question of prezygotic and postzygotic barriers. To study the postzygotic barrier, we focused on the diversity of endosymbiotic bacteria known in B. tabaci: one primary symbiont and six secondary symbionts. For this purpose, we established a multiplex PCR in order to detect the endosymbiotic community in the wild population of B.tabaci: Hamiltonella, Rickettsia, Cardinium, Arsenophonus and Wolbachia. Some of these symbionts are well known in other insect's taxons to play an important role by cytoplasmic incompatibilities, probably enhancing strong genetic diversity. Our results are discussed, correlatively to genetic diversity analyses produced by nuclear (microsatellites) and mitochondrial (COI) data.