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In Mozambique, at least two different phytoplasmas induce lethal yellowing type syndromes in coconut palms

Dollet M., Lourenço E., Macome F., Vaz A., Fabre S.. 2012. In : IOM. 19th Congress of the International Organization for Mycoplasmology (IOM 2012), 15-20 July 2012, Toulouse, France. s.l. : s.n., 1 p.. Congress of the International Organization for Mycoplasmology. 19, 2012-07-15/2012-07-20, Toulouse (France).

Several African lethal yellowing type syndromes (LYTS) are similar to “Coconut Lethal Yellowing” (CLY) in the Caribbean that affects coconut palms. In West Africa, the phytoplasmas associated with LYTS in Ghana – Cape Saint Paul Wilt Disease (CSPWD) were different from those of the 16SrIV subgroups associated with CLY. In East Africa a LYTS called “Lethal Disease” occurs in Tanzania (LDT). Recent studies along the coast revealed five LDT genotypes distributed into two groups (Northern and Southern groups). The phytoplasmas associated with the LYTS in Mozambique were claimed to be closely related to the CSPWD phytoplasmas and different from those causing LDT. We sampled several coconuts with LYTS in Northern Mozambique in order to validate the observation. Sampling was performed in Zambesia around Quelimane – from Macuze to Inhassünge. In Cabo Delgado province, samples were collected from Mecufi – 40 km South of Pemba – to Palma, 160 km North of Pemba, 20 km South of Tanzania. Samples included petioles of inflorescences or trunk borings. DNA extraction was done with CTAB. PCR were performed with P1/P7 primers or Rohde's primers claimed to be specific for LDT. PCR products were cloned in pGEM-T. DNA sequences were submitted to a BLAST search. Around 90% of the extracted DNAs were amplified with P1/P7. Only the samples from Nanjaba, a village 170 km south of the Tanzania border, were amplified with Rohde's primers. BLAST search with the sequences obtained in Nanjaba revealed 100% similarity with phytoplasmas of the Southern group, genotype V, from the South of Tanzania. Other samples, closer to the border of Tanzania (in Palma), showed sequences similar to the phytoplasmas found in Zambesia, which were closer to CSPWD phytoplasmas. These results raise the question of transmission of these two phytoplasmas present in a same region in Mozambique.

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