The aim of this study was to determine the 25-hydroxyvitamin D3 (25-OH-D3) amounts in meat and serum of the Moroccan one-humped camels. The obtained results showed that the 25-OH-D3 amounts in serum (ng/mL), liver, kidney and muscle (ng/g) were 390±45; 7.071±1.003; 6.154±1.067 and 4.241±1.045 respectively. Values in liver were significantly higher (p< 0.05) than those of muscle. Serum 25-OH-D3 levels in camels were very higher than those of ruminant species. Meat of camel was rich in water and ash, and the amounts of 25-OH-D3 in it's various analyzed tissues were similar to those reported for this constituent in the corresponding tissues of bovine species and slightly higher than those measured in other domestic animals.