Soil contamination by trace elements may occur afte r recycling organic waste on agricultural lands. This paper focused on the impact of trace elements and soil chemical properties in rhizotoxicity in soils amended with organic wastes. An ecotoxicologi cal approach using biological tests was carried out in order to ease the determination of the effec ts of trace elements contaminated soils on the root elongation. Our results revealed that (i) the degre e of rhizotoxicity was depending on the type of organic wastes and the application rate, (ii) the c orrelation between root elongation and Cu in soil solution was not significant. This first assessment suggested the complex interac tion between trace elements and soil chemical properties.