OSB panels were manufactured with a mixture of pine and cypress heartwood and lignin and tannin based resins in order to propose an eco-friendly wood composite. The resistance of OSB panels against Reticulitermes santonensis was tested according to EN 118 and EN 117 as well as in a field test. OSB made from cypress showed more resistance against the tested termite, the resistance decreased as the percentage of pine increased. The degree of attack differed according to the choice of standard procedure. The field test results revealed that in outdoor conditions and when the termites had a feeding choice, all mixtures showed the same behaviour. The decay resistance was assessed according to EN 12038, and the results revealed that the resistance increases when the percentage of cypress increases.