Among hematophagous arthropods, ticks transmit the greater variety of pathogens of public health and veterinary importance. Due to socio-economic and environmental factors, such as human practices, increased travel, global market, global warming, and environmental changes, the incidence of tick-borne diseases in both humans and animals is increasing worldwide, leading to a need for extended surveillance tools. Recently, in Europe a large scale epidemiological study was conducted on 19,474 Ixodes ricinus nymphs collected from five European countries using a powerful new high-throughput approach to screen tick-borne pathogens (Michelet et al., 2014). The technology used in this study is a microfluidic high-throughput Taqman realtime PCR (BioMarkTM dynamic arrays, Fluidigm Corporation), allowing the simultaneous detection of 25 bacterial, 12 parasitic and 22 viral species across 94 samples of ticks. They successfully determined the prevalence of expected tick-borne pathogens (Borrelia burgdorferi sensu lato, Babesia divergens, Tick-Borne Encephalitis virus, etc.), unexpected (Borrelia miyamotoi, Nairo-like virus) or rare (Bartonella henselae, Eyach virus) tick-borne pathogens. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens in various samples, and which can also be customized for the survey of emerging diseases in different areas of the world. Caribbean, are a risk area for the (re)-emergence of vector-borne diseases. Population in Caribbean is in expansion, leading to a growing food demand. Maintaining a healthy livestock industry is crucial but often difficult to manage because of tick-borne diseases such as anaplasmosis, ehrlichiosis, or babesiosis. The Caribbean is also a world interface, with numerous air and maritime networks (tourism, and animals trade) leading to a serious risk of dispersion of tick and their tick-borne pathogens in this area. Moreover, few repo