This research was conducted in 2015 to determine the Chloropidae fauna of sugarcane fields in Khouzestan Province (Iran). Chloropid flies were collected using sweeping nets before harvesting of sugarcane varieties. As a result of this survey 10 species from two subfamilies were identified: Aphanotrigonum cinctellum (Zetterstedt 1848) and Oscinella pusilla (Meigen 1830) (subfamily Oscinellinae); Lasiosina herpini (Guérin-Méneville 1843), L. paralittoralis (Dely-Draskovits 1981) Meromyza nigriventris (Macquart 1835), Thaumatomyia sulcifrons (Becker 1907), T. notata (Meigen 1830), T. glabra (Meigen 1830), Platycephala umbraculata (Fabricius 1798) and P. rugosa (Nartshuk 1964) (subfamily Chloropinae). P. umbraculata and P. rugosa are new for Iran fauna and all species are new to Khouzestan Province. Besides them, we identified two genera Conioscinella sp. and Elachiptera sp.