Sugarcane yellow mite, Oligonychus sacchari (Prostigmata: Tetranychidae), is one of the main detrimental pests in sugarcane growing areas in Iran. The early populations of mites colonize on the lower surfaces of sugarcane leaves. Because of high reproductive potential, multi-generation are produced per year under warm and humid temperature during sugarcane growing season. This pest imposes economic damage to sugar industries. Field trials were conducted on evaluation of varietal resistance of five sugarcane varieties against yellow mite during two successive seasons 2014–2015 and 2015–2016 in sugarcane fields at Salman Farsi Agro-industry unit in Ahwaz, Iran. After initiation of O. sacchari activity in fields, in each plot, fifteen leaves were selected at random and number of living mites was recorded. The results showed that there were significant differences among tested varieties. The variety IRC99-02 was the most susceptible in tested years by CP57-614 in the total number of living mites and dry-leaf even though CP69-1062 was the most resistant variety.