Worldwide, native grasslands are being converted to non-native pastures and cropland. This process threatens local grassland biomes as well as the livelihoods of farm families that utilize these grasslands. In the Río de la Plata grasslands region meat production and multispecies native grasslands have coexisted for more than 400¿years. Low levels of meat productivity and farm income, however, trigger replacement of native grasslands by crops and leys and threaten the survival of local beef farming systems. We studied the economic and environmental performance of beef farming in the region based on interviews and field measurements on 280 case study farms with the following aims: (a) to identify the multi-functional economic and environmental performance of beef farms across the Rio de la Plata grasslands biome; (b) to identify farms with 'outstanding' multi-functional performance; (c) to compare performance levels with those found in other regions; and (d) to discuss the implications of the outstanding farms for the development of new systems of meat production. The representativeness of the case study farms was ascertained by comparing them with a farm typology constructed from survey data of 15,448 beef farms situated predominantly on native grasslands in Argentina, Brazil and Uruguay. We identified seven farm types on the basis of farm size, labour, farm specialization, land use and stocking rate. We identified positive deviant farms based on Pareto-ranking and compared these with a classification based on threshold values provided by experts. Out of the 280 farms, 41 were ranked as Pareto-optimal, i.e. outperformed other farms in one or more indicators without being outperformed in other indicators. Out of these, 5 were positive deviants, achieving on average 192¿kg¿LW¿ha-1¿yr-1 of livestock productivity and 201 US$ ha-1¿year-1 farm income, having most favourable values for fossil energy consumption, phosphorus balance, carbon footprint and having over 95% of t