For the implementation of the sequential aerosol technique (SAT), several technical constraints such as the calibration of the spray, as well as the meteorological conditions during spray operations, need to be taken into account [1, 2]. Because some of these constraints differ depending on whether savannah or riverine tsetse flies are the target of control, the technique, which has been proven successful in southern Africa against Glossina morsitans, cannot be transferred indiscriminately to combat riverine tsetse flies [3]. This paper aims at addressing these elements and identifying which studies are required before using SAT against riverine tsetse species.