Tsetse flies (Glossina), are African hematophagous Diptera, main vectors of animal and human trypanosomoses in Africa. The Sterile Insect Technique (SIT), has been success-ful to control tsetse when conducted on isolated populations. A new approach, called boosted SIT, combining SIT with the contamination of wild females by sterile males coated with pyriproxyfen (PP) has been proposed for large-scale control of vector pop-ulations. The aim of the study was to determine in laboratory conditions the transfer dynamics of PP from a sterile male to a female during mating, as well as the impact of PP on female fertility. To do this, we developed a method of treating sterile males by impregnating them with a powder containing 40 % of PP. The results showed that the PP had no impact on the survival of sterile males. With respect to persistence, PP per-sisted on sterile males up to 10 days at a dose of 100 ng. In addition, we showed a hor-izontal transfer of PP from a sterile male treated to a female. During mating, females received an average of 50 ng PP and by simple contact without mating more than 10 ng PP. Ongoing experiments will verify the impact of the transferred PP on female fertility. These initial results must be confirmed under field conditions. Eventually, the large-scale application of the boosted SIT approach against tsetse flies could be implement-ed in Africa. This study was carried out and financed within the framework of the REVOLINC project (ERC Consolidator grant no. 682387)