To determine if African swine fever (ASF) is maintained in a sylvatic cycle in Mozam-bican wildlife areas, we investigated the presence of soft tick reservoirs of ASFV and their infection rates in the Gorongosa National Park (GNP) and its surrounding villages. The GNP is one of the largest national parks located in the centre of the country. A total of 1,658 soft ticks were recovered from warthog burrows and pig-pens at the wildlife/livestock interface and viral DNA was detected by nested PCR in 19 % of Ornithodoros porcinus porcinus and 15 % of O. p. domesticus. Isola-tion of the virus was successful in approximately 50 % of the PCR-positive samples with nineteen haemadsorbing virus isolates recovered. These were genotyped using a combination of partial gene sequencing (p72, p30 and p54) and analysis of the cen-tral variable region (CVR) of the B602L gene. Eleven isolates clustered in genotype II and were homologous to contemporary isolates from southern Africa, the Indian Ocean and Eastern Europe. Three isolates grouped within genotype V were simi-lar to previous isolates from Mozambique and Malawi. The remaining ¿ve isolates constituted a new, previously unidenti¿ed genotype, designated as genotype XXIV. The isolates classi¿ed within genotypes II and XXIV clustered into three major sub-types based on their p30 and p54 sequences. Based on analysis of the CVR gene, the viruses were classi¿ed into eight subgroups. This work corroborates for the ¿rst time the wildlife origin of Genotype II and con¿rms that a large diversity of ASFV strains maintained in wildlife areas can act as a permanent source of virus for the domestic pig value chain in Mozambique and beyond its boundaries. Their genetic similarity to ASFV strains currently spreading across Eastern Europe suggests that the African sylvatic cycle has a high potential to disseminate different ASFV into new geographic areas and justi¿es the need to continue studying the epidemiology and ecology of ASF sylvatic host