The genus Capulavirus has been proposed to accommodate two divergent geminiviruses, namely Euphorbia caput-medusae latent virus (EcmLV) from South Africa and French bean severe leaf curl virus from India (FbSLCV; Bernardo et al. 2013). Since then, two other capulaviruses have been discovered, Alfalfa leaf curl virus (ALCV) from France and Spain, and Plantago lanceolata latent virus (PlLV) from Finland (this conference, Bernardo et al. 2016). The Capulavirus genus was initially defined by genome relatedness and genome organization, and recently a potentially third distinguishing genus-level feature was added. Indeed, ALCV was the first geminivirus for which an aphid transmission was demonstrated (Roumagnac et al. 2015); the aphid vector belongs to the species Aphis craccivora. Using an agroinfectious clone of ALCV and a reared population of A. craccivora, the features of the aphid transmission are under investigation. To further validate aphid transmission as an additional feature to distinguish capulaviruses from other geminivirus genera, the potential aphid transmission of a second capulavirus, namely EcmLV is under investigation. In this attempt, we have collected and reared the most common aphids observed on Euphorbia caput medusae plants, which belong to the species Aphis tirucallis Hille Ris Lambers, 1954. A. tirucallis aphids have tiny differences of morphology with A. craccivora aphids and were not distinguishable from them according to CO1 sequences.