The concept and methods to assess soil quality have been widely debated in the literature for the last twenty years. We developed a new framework to assess soil quality following an integrative approach based on the measurement of soil dynamic functions rather than stocks, namely Biofunctool®. Biofunctool® accounts for the interactions between soil physico-chemical properties and soil biological activity. It consists of twelve in-field, time- and cost- effective indicators to assess three main soil functions: carbon transformation, nutrient cycling and structure maintenance. Firstly, the capacity of Biofunctool® to assess the impact of land management on soil quality was validated through a reliability, redundancy and sensitivity analysis. The results over 250 sampling points in Thailand showed the relevance of each of the twelve indicators to assess soil functioning. Secondly, we applied Biofunctool® and aggregated the indicators in a Soil Quality Index that synthetize the impact of land management on soil quality. Biofunctool® was applied within various contexts (tree plantations, agroforestry, conservation agriculture etc.) and two cases study will be presented: i.) impacts of a disturbance gradient based on various land uses and rubber tree stands in Thailand ii.) impacts of conservation agriculture practices in Cambodia. The overall results proved that Biofunctool® index provides a synthetic soil functioning score that is sensitive to land management and is robust in various pedo-climatic contexts. Therefore, Biofunctool® is a reliable tool to assess the soil integrated functioning, i.e., soil quality, and could be included within larger environmental impact assessment frameworks.