The moth stalk borer Chilo sacchariphagus is the most destructive insect pests limiting the production of sugarcane in Guangxi province, China. Current control strategies are mainly focused to the application of chemical insecticides. During 2016-18, the occurrence of the overwintering generation of C. sacchariphagusand its egg parasitoid wasps Trichogramma chilonis and Telenomus dignus were investigated. Findings showed that the overwintering generation of C. sacchariphagus began to emerge in early March and the peak of adult emergence was from the mid-March to early April. At the beginning of peak period, fewer egg masses were laid by C. sacchariphagus and percentage of egg parasitism by parasitoid wasps was low. At the peak period of C. sacchariphagus, more egg masses were laid and parasitism level increased. Both Telenomus dignus and Trichogramma chilonis are dominant natural enemies in Guangxi, which play an important role to mitigate population level of stalk borers. The seasonal dynamic of egg parasitoids' parasitism was typically adapted to the natural enemy following-up pattern. The percentage of parasitism was lower at the early stage of sugarcane growth from March to April and higher (up to 90%) at the late growth stage in September which played an important role in controlling Chilo sacchariphagus.