The trait-based approach can address questions in order to understand how the functioning of organisms scales up to that of ecosystems and controls some of the services they deliver to humans, including in agriculture. However, the importance of interspecific vs the intraspecific trait variability (ITV) for classifying species according to their traits in agrosystems on a large diversity of pedoclimatic situations and cropping systems remains still open. Here, we addressed three questions: How do measured traits vary across years and seasons? Are species rankings conserved across years and season? And which traits and species are the more stable and repeatable for sown and spontaneous species? We conducted a two-year experiment in a vineyard, and we measured four leaf and plant functional traits of 14 sown species and 43 spontaneous species that grew among sown species. Traits were measured at two key phenological stages for grapevine: budburst and flowering during two successive years with contrasted rainfall (2017 and 2018). We studied seasonal and interannual trait variations, rankings between species, and variance partitioning. The species factor explained the greatest part of trait variations across years and seasons. Sown and spontaneous species traits varied in the same way, and traits related to plant dry matter contents were the more stable across periods. Moreover, species rankings were conserved across years and seasons for all traits except plant height. Sown species showed better ranking conservation than spontaneous species overall. The trait-based approach seems promising for the comparison of various cropping systems involving sown and spontaneous species, and may help identifying service crop species related to specific agroecosystem services. Further research is needed to bring more knowledge on trait variations under a diversity of agrosystems, and to improve theoretical frameworks that would help the design of sustainable agrosystems that provide