Transverse modal analysis of timber panels is a proven effective alternative method for approximating a material's elastic constants. Specific testing configurations, such as boundary conditions (BC) and location of sensor and impact, play a critical role in the accuracy of the results obtained from the experimental assessment. This article investigates signal-specific details, such as the signal quality factor, that directly relate to the damping properties and internal friction as well as frequency shifting obtained from six different BCs. A freely supported (FFFF), opposing minor sides (shorter length) simply supported, and major sides (longest length) free (SFSF), as well as the reverse of the SFSF configuration with minor sides free and major lengths simply supported (FSFS) and all sides simply supported (SSSS) setup, are investigated. Variations into the proposed methods used to achieve an FFFF supported system are also considered. A combination of experimental testing in parallel with finite element analysis was conducted to re-create the setup that would be used within a manufacturing facility for nondestructive assessment of full-size cross-laminated timber panels. The differences between all BC configurations for their resonance frequency quality and location indicate that a freely supported system provides higher-resolution results, good comparison of less than 10 percent error with the finite element analysis and experimental results, and advantages in a simple experimental setup for the intended application.