RATIONALE - Previous studies have shown that new F1 hybrids of Coffea arabica produce higher yield than commercial varieties in both full-sun and agroforestry systems (AFS). However, the physiological and molecular mechanisms controlling their improved performances are still largely unknown. Different water use strategies, such as a trade-off between maintaining photosynthesis while reducing water loss, have been described in drought resistant varieties. We hypothesize that a capacity to better regulate photosynthesis and water loss allow F1 hybrids to recover quicker from the dry season. To test this hypothesis, a field trial submitting these hybrids to different water regimes was set-up in the NOMAFSI station (Son La). METHODS - As part of the H2020 BREEDCAFS European project, Starmaya, Centroamericano (H1) and Mundo Maya (H16) F1 hybrids, along as Marsellesa and a local Catimor pure lines used as controls, were planted (2018) in AFS with Leucaena leucocephala and subjected to three water treatments: rainfed, water-suppressed and irrigated. The experiment followed a 3x4 design with the three treatments repeated in 4 blocks. Each block included a row of 8 plants by accession. Probes were installed in trunks to constantly monitor sap-flow. Photosynthesis, stomatal conductance, water potential, leaf area and dry matter content were also measured regularly. Plant reproductive and vegetative growth (fruit number, height, trunk diameter, number of nodes…) were periodically monitored since May 2019. Yield and fruit/leaf area ratio were assessed in 2020. Climate conditions and soil moisture were also monitored. RESULTS - Irrigated plants had significantly higher leaf water potential, conductance and photosynthesis compared to those rain-fed and water-suppressed. For all agronomic traits, the lowest values were observed for control Catimor. For all accessions, sap-flow, photosynthesis, and conductance dropped down during the dry season in a similar way. However, daily sap-