The proposal of a model of development that reconciles environmental conservation, especially of forest resources, and socioeconomic development is still a challenge to be achieved worldwide, especially in the Amazon region. Due to its amplitude, the Brazilian portion of the Amazon is a matter of great concern nationally and internationally. Agriculture stands out in the Brazilian Amazon for its socioeconomic importance and its tremendous potential to alter ecosystems and the provision of ecosystem services (ES). Among the different forms of agriculture (i.e. large and small farmers), family farms are key players for promoting rural sustainable development. Despite its importance, local actors supporting family farms face numerous challenges to promote agroecological transition of these farms. The general question that we want to contribute to with this thesis is: How can the conceptual framework of ES serve as a cognitive and operational basis to support the agroecological transition? We carried out this research in two contrasting municipalities in the eastern part of the Brazilian Amazon, Irituia and Paragominas. The farming sector of the first study site is dominated by family farming, which is often biodiversified. Contrastingly, large-scale industrial agriculture predominates in the second study site, although it coexists with family farming. We adopted a multi-actor perspective, with the participation of a heterogeneous set of local actors (e.g. policy makers, researchers, rural extension agents, farmers) related to rural issues. We implemented a “mixed methods approach” combining well-tested qualitative methodologies, such as semi-directive interviews and participant observation, with semi-quantitative methodologies such as questionnaires and a role-playing game. We first aimed to understand the perception of different local actors about ES and their co-production process. We observed that, in general, a diversity of ES is perceived by local actors. The perc