Monitoring the fiber quality preservation in industrial ginning plants avoids the losses that may arise due to improper settings along with wear and tear of machineries. For this purpose, fibre quality parameters obtained from the ginning plants can be compared with that of micro-gins by processing same cotton lot. However, users and stakeholders remain in state of dilemma with respect to performance of microgins to preserve the fiber quality due to the lack of reference material. Hence, there is a need to develop standard seed cotton reference material with known fiber characteristics in order to perform periodical checks of the micro-gins. In this work, a reference material was developed and measured for its homogeneity. Study displayed that that reference material is suitable for the micro gins employed to monitor the fiber quality preservation performance of industrial plants.