To document and halt biodiversity loss, monitoring, quantifying trends and assessing management and conservation strategies on wildlife populations and communities are crucial steps. With increasing technological innovations, more and more data are collected and new quantitative methods are constantly developed. These rapid developments come with an increasing need for analytical skills, which are hardly accessible to managers. On the other hand, researchers spend more and more time on research grant applications and administrative tasks, which leaves fewer opportunities for knowledge transfer. This situation tends to increase the gap between researchers and managers. Here, we illustrate how to fill this gap by presenting two long-term collaborations between a research unit—Centre for Functional and Evolutionary Ecology; CEFE—and a national agency—French Biodiversity Agency; OFB. The first example is a collaboration providing statistical support to national parks for the design and implementation of scientific monitoring protocols. It relies on the recruitment of a research engineer funded by OFB and physically based at CEFE, who works closely with OFB and managers. The second example is a collaboration on the management of large carnivores. For more than 10¿years, it has involved several PhD students and post-doctoral fellows co-supervised by CEFE and OFB, and has recently resulted in the recruitment of a permanent OFB researcher who works half-time at CEFE and half-time at OFB. These case studies illustrate the modalities of collaborative work between public institutions acting at different levels of biodiversity conservation for the co-construction of research agendas and the exchange of knowledge. These collaborations also bring out some challenges. Inter-knowledge and mutual learning remain difficult at scales larger than that of the teams concerned. The staff working at this interface needs to possess good listening skills, respect all partners' needs and demo