This document summarizes the activities and results of a second participatory workshop to “Co-build a One Health community of practice in the Caribbean”, held in Punta Cana, Dominican Republic on November 20th-23rd, 2023. The participatory workshop is part of the AUSCAR (French acronym for One Health approach to reduce health risks in the Caribbean) research project funded by the INTERREG Caribbean program (https://interreg-caraibes.eu/) coordinated by the CIRAD in partnership with CARIBVET, CPHD, OIRSA, AGROSAVIA, CATIE, INIVIT, IIFT, and CORBANA. The participatory workshop was organized by CIRAD (French Agricultural Research Centre for International Development). During the four-day event, 41 participants from animal, plant, environmental and human health and social sciences collectively build the theory of change of the One Health community of practice in the Caribbean through a participatory strategic planning process, by using the ImpresS ex ante approach (https://impress-impact-recherche.cirad.fr/). This document presents the results of this process: Vision of the future, Problem tree, Actors mapping, Outcomes mapping, Consolidation of the outcomes mapping and identification of strategies.