Purpose: Anthropogenic activities are a major driver of soil and land degradation. Due to the spatial heterogeneity of soil properties and the global nature of most value chains, the modelling of the impacts of land use on soil quality for application in life cycle assessment (LCA) requires a regionalised assessment with global coverage. This paper proposes an approach to quantify the impacts of land use on soil quality, using changes in soil organic carbon (SOC) stocks as a proxy, following the latest recommendation of the Life Cycle Initiative. Methods: An operational set of SOC-based characterisation factors for land occupation and land transformation were derived using spatial datasets (1 km resolution) and aggregated at the national and global levels. The developed characterisation factors were tested by means of a case study analysis, investigating the impact on soil quality caused by land use activities necessary to provide three alternative energy supply systems for passenger car transport (biomethane, ethanol, and solar electricity). Results obtained by applying characterisation factors at local, regional, and national levels were compared, to investigate the role of the level of regionalisation on the resulting impacts. Results and discussion: Global maps of characterisation factors are presented for the 56 land use types commonly used in LCA databases, together with national and global values. Urban and industrial land uses present the highest impacts on SOC stocks, followed by severely degraded pastures and intensively managed arable lands. Instead, values obtained for extensive pastures, flooded crops, and urban green areas often report an increase in SOC stocks. Results show that the ranking of impacts of the three energy systems considered in the case study analysis is not affected by the level of regionalisation of the analysis. In the case of biomethane energy supply, impacts assessed using national characterisation factors are more than double thos