The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly, cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species. Wacapou (Vouacapoua americana., Fabaceae) is a well-known Guianese wood species commonly used in local wood construction due to its outstanding natural durability, which results from the presence of a large panel of extractives compounds. In addition, its industrial processing generates large amounts of residues. Wacapou residues were extracted by maceration using four different solvents (water/ethanol, ethyl acetate, hexane and dichloromethane/methanol), separately and successively. The yield of each extractive fraction was determined, and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS). Ethyl acetate led to the highest extraction yield, and the active compounds were identified in the obtained extractive fraction. In this sense, the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory (with temperate and tropical microorganisms), according to the solution concentration (1%, 2.5%, 5%, 8% and 10%). Finally, Virola michelii Heckel wood samples (low durable species) were impregnated with the 8% concentration solution. The impregnated wood samples were then exposed to a soil bed test. The results highlighted that the nature of the solvent used during wood maceration affects the content of the obtained extractive fractions. Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry (UHPLC-HRMS) analyses showed the influence of extraction parameters on the nature of the extracted molecules. Wacapou extracts (from ethyl acetate maceration) showed good anti-fungal and anti-termite activities. Additionally, the concentration in extractives had an impact on the anti-termite activity level for Reticulitermes flavipes