Cultivar and geographic location could have an impact on bacterial and fungal communities associated with the mango surface (Mangifera indica). Microbial communities on the surface of two varieties of mangoes ('Cogshall' and 'José') were characterized in two different locations in Reunion island (St-Gilles and St-Pierre). The bacterial phyla Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria were the most dominant in all samples, with 85.03, 8.17, 4.15, 1.02 and 0.47% in 'Cogshall', and in 'José' samples the proportions were 74.81, 11.05, 7.79, 3.24 and 0.81%, respectively. Similarly, Ascomycota, Basidiomycota and Chytridiomycota were the most abundant fungal taxa in all samples, with 88.40, 11.57, and 0.03% in 'Cogshall', and in 'José' mango samples the proportions was 90.2, 9.79, and 0.01%, respectively. Alpha and Beta diversity analyses revealed the presence of different bacterial and fungal populations in Cogshall and José cultivars. In addition, significantly different populations were detected on 'Cogshall' of St-Gilles and St-Pierre. However, no significant difference was observed in the other samples, suggesting that the cultivar had less impact on microbial communities relative to the geographic location. Results of the present study represent an advancement of the current knowledge on the bacterial and fungal microbiome on mango surface.