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Impact of sterile Aedes aegypti males releases on vector dynamics: Insights from Malaysian field trials

Nazni W.A., Guat Ney T., Nuradila M.A., Hakimi S.I.S.N., Tanusshni M., Arif M.A.M., Hanini A.N., Shazia I.A., Tan A.M., Rabizah H., Zainuri M.D.A., Hadi A.H., Norazah A., Lee H.L., Maiga H., Bouyer J., Cheong Y.L.. 2025. Infectious Diseases of Poverty, 14 : 16 p..

DOI: 10.1186/s40249-025-01303-x

Background: The Sterile insect technique (SIT) has been successfully used in agricultural pest control, leading to interest in its application for public health, particularly in controlling Aedes mosquitoes in the USA, Italy, Cuba, and Greece. Malaysia has conducted a small-scale SIT pilot trial since 2019 for dengue control. This study evaluates mosquito populations in treated and untreated sites through three objectives: (1) comparing mean larvae per trap (MLT) and dengue cases for Ae. aegypti and Aedes albopictus; (2) estimating survival rates and wild populations using mark-release-recapture (MRR); and (3) analysing spatial distribution in treated and untreated sites. Methods: Ae. aegypti males, irradiated at 55 Gray, were released in three locations: Pangsapuri Kota Laksamana (KT), Malacca (19 months), Pangsapuri Taman Tasik Utama (TTU), Malacca (8 months), and the Customs, Immigration, and Quarantine Complex (CIQ), Johor (7 months). Statistical analyses assessed SIT effectiveness, including T-tests for larval density and ovitrap indices, Mulla's formula and relative variance (RV) for population reduction, and the Lincoln Index for estimating wild male populations and probability of daily survival. Results: Weekly releases of sterile Ae. aegypti males at doses of 1278–7942 males/ha achieved a sterile-to-wild male ratio of 5.85 and a mean daily survival rate of 0.61, leading to significant reductions in larval densities: 76.25% in Kota Laksamana (KT), 96.74% in Taman Tasik Utama (TTU), and 89.00% in CIQ Gelang Patah, thereby supporting dengue control efforts. In KT, the MLT was initially low but increased, although with suppression¿<¿90%, there was a reduce of dengue cases throughout the release period. The MRR's mean survival rate (±¿standard deviation) in KT was 0.61 (±¿0.08). The spatial clustering of Ae. aegypti was observed in central blocks during the high MLT period. However, larval densities rebounded after releases ceased. Spatial clustering revealed no

Mots-clés : lâcher d'insectes stériles; aedes aegypti; aedes albopictus; vecteur de maladie; contrôle de maladies; lutte anti-insecte; piège; distribution spatiale; italie; malaisie

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