Agroecological transition (AET) of agrifood systems is a multidimensional process involving diverse stakeholders. Participatory innovation and co-creation of knowledge are also at the heart of agroecology. Deliberately designed Multi-stakeholder platforms (MSPs) are collaborative spaces conducive to driving this type of collaboration and change. However, how these processes are operationalized is poorly documented. This study contributes to filling this gap by highlighting how MSPs can be important vehicles for enabling AETs. As part of the CGIAR Initiative on Agroecology, Agroecological Living Landscapes (ALLs) were set up as MSPs specifically focusing on co-creation of agroecological innovations. By examining different ALL configurations in seven countries, we analyze MSP attributes that can enable and shape AETs at different stages or “levels” of the transition pathway. We find that MSPs can advance different levels of the transition pathway simultaneously by working on multiple innovations involving various stakeholder coalitions. MSPs navigate complex environments, with diverse stakeholders playing multiple policy and non-policy roles to support AETs. The results also illustrate the intersection between MSP configuration and AET objectives, such as policy change, agroecology market support, and scaling adoption. Finally, we discuss the non-linear nature of AET pathways and political economy considerations for stakeholder engagement in MSPs.