In May and October 2023, unusual virus-like disease symptoms of mosaic on leaves and brown spots on fruits were observed on cherry tomato (Solanum esculentum, cv. Tutti Frutti) and ancient tomato (cv. Coeur de boeuf) varieties in greenhouses located in the localities of St. Pierre and St. Louis in the South of Reunion Island. The recent description of emerging tobamoviruses on solanaceous plants in several parts of the world (Salem et al. 2023), with the first descriptions of tomato mottle mosaic virus on tomato in the sister island Mauritius (Maudarbaccus et al. 2021) and tobacco mild green mosaic virus on eggplant in Reunion (Lett et al. 2024), suggested the possible involvement of a tobamovirus. To identify the potential agent(s) causing these symptoms, leaf samples from 12 symptomatic plants were collected, dehydrated, and subjected to total RNA extraction using the RNeasy Plant Extraction Kit (Qiagen, France). The presence of tobamoviruses was tested by reverse transcription (RT)-PCR using generic primers designed to amplify a fragment of the RNA-dependent RNA polymerase (RdRp) of tobamoviruses (Li et al. 2018). RT-PCR amplicons of the expected size (880 bp) were obtained from the 12 samples and subjected to direct sequencing in both directions (Macrogen Europe, Amsterdam, The Netherlands). No PCR amplicons were obtained from the negative controls. BLASTn analysis showed that all 12 assembled nucleotide sequences (GenBank accession nos. PQ505139 to PQ505150) were very similar to each other (>99.2%) and shared 98.2 to 99.3% identity to tomato mosaic virus (ToMV) genome sequences (GenBank accession nos. OQ722320, ON987477, DQ873692, PP856214, and AB355139). To confirm this diagnostic, the specific primers ToMV-F5130 and ToMV-R5928 were designed on the reference sequences ON987477 and AB355139 for the amplification of a 743-bp product covering a region overlapping the movement protein and capsid protein regions. Amplicons of the expected size were obtained from th