Sugarcane (Saccharum interspecific hybrids) is a major crop contributing to both sugar production and economic stability, but various pathogens threaten its production. Unusual chlorosis was observed on several plants of variety R570 in a field trial established on 5,000-m2 to investigate the impact of sugarcane mosaic virus and the leaf scald pathogen (Xanthomonas albilineans) on the microbiome of sugarcane varieties at Canal Point, FL. Disease (chlorosis) incidence in R570 rose from 6% in August 2023 (plant cane) to 28% by April 2025 (second ratoon). A total of five samples were collected from five plants showing chlorosis. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). For high-throughput sequencing (HTS), five single-indexed, ribosomal RNA-depleted cDNA libraries were prepared using the TruSeq Stranded Total RNA Library Prep Plant Kit (Illumina, San Diego, CA). Sequencing was done on the Illumina NextSeq 2000 platform using a P2 100-cycle kit, yielding 11.12 to 21.49 million single-end reads. HTS reads were analyzed using PhytoPipe (Hu et al. 2023). BLASTn analysis of five assembled viral contigs (ranging from 2,091 to 3,149 nucleotides (nt); GenBank accessions PX394828 to PX394832) resulted in the identification of Ethiopia maize-associate virus (EMaV; MF415880) as the top hit, with over 95% genome coverage and 82% nucleotide identity. Phylogenetic analysis using published umbra-like viruses (ULVs) confirmed that the sugarcane virus clusters with EMaV originally identified in maize in Ethiopia (Adams et al. 2017). Open reading frame (ORF) analysis of the derived viral sequence identified four ORFs, consistent with those of other Class 2 ULVs infecting monocots (Ying et al. 2024). The inferred amino acid sequences exhibited 84% similarity with the RNA-dependent RNA polymerase (RdRp) of EMaV. Additionally, a novel small ORF (180 nt) was identified in the 3' UTR, absent from all known ULV sequences (Simon et al. 2024). Two pairs