Oil was extracted from Nile perch (Lates niloticus) head collected from the Victoria Lake (Uganda). Oil content was in the range 15-18% of dry weight. The predominant fatty acids found in crude oil were 14:0, 16:0, 16:1, 17:0, 18:0, 18:1, 18:2, 18:3, 20:4, 20:5, 22:5 and 22:6. The crude Nile perch oil contained a substantial amount of oleic acid (15.2 mol%) and an appreciable level of combined n-3 polyunsaturated fatty acids (16.2 mol%). Comparing with the commercial production of fish oils, oil from Nile perch heads might stimulate its future use for human consumption and easily could be valorized.