Using elicitors is a way of reducing the use of weedkillers, fungicides and insecticides by inducing the natural defence reactions of plants. FEN560 is a natural product extracted from Fenugreek (Trigonella foenum-graecum) and its elicitive action is currently known on many pathosystems. Coffee Berry Disease (CBD), due to Colletotrichum kahawae, causes fruit-rot (Coffea arabica) and can reduce yields by up to 40%. The objective of the study was to characterize the reactions induced by Colletotrichum kahawae on the Coffea arabica varieties Caturra (susceptible) and Marseilleissa (low susceptibility) and to verify the action of a natural elicitor (FEN560) on that pathosystem. The enzyme activity of phenylalanine ammonia-lyase (PAL) and the caffeine and chlorogenic acid (CGA) concentrations in hypocotyls of seedlings inoculated with the pathogen, then treated with FEN560, were quantified. The inhibitory effect of caffeine on C. kahawae was confirmed. Direct observation of the symptoms on seedlings demonstrated an effect of FEN560 on the studied pathosystem. However, in spite of the positive action of the elicitor, it did not seem to stimulate the biosynthesis of alkaloids (caffeine) or of polyphenols (CGA). Nevertheless, the pathogen triggered synthesis of caffeine and chlorogenic acid in Coffea arabica, which thus appeared to be worthwhile markers for studying Coffee Berry Disease resistance in Coffea arabica.