Fourteen strains of Enterococcus species, 3 strains of Lactobacillus species and two strains of Streptococcus isolated from 6 different gariss samples collected from Kordofan and Khartoum production sites, Sudan were screened for antibacterial activity against Staphylococcus aureus ATCC 25923 and E.coli ATCC25922 and for their antibiotic resistance against 9 antibiotics. The results indicated that all the 19 strains of LAB isolated from gariss were found to show no antimicrobial effect on both Staphylococcus aureus ATCC 25923 and E.coli ATCC25922. As intrinsic resistance of Enterococci for antibiotics, there were 57.14% (8 isolates) showing resistance to at least one antibiotic and some even to almost all antibiotics studied, one strain (E. durans R05) resistant to penicillin. S. acidominimus was resistant to only Ciproflaxin while S. thermophilus was resistant to Chloramphenicol, Erythromycin and Ciproflaxin; L. plantarum BJG32 was resistant Chloramphenicol, Erythromycin and Ciproflaxin; L. pentosus S2LPO2 was resistant Erythromycin and Ciproflaxin, while L. plantarum BJ6 was not resistant to any one of antibiotics investigated.