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Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11

Chan Salum T.F., Villeneuve P., Baréa B., Yamamoto C.I., Côcco L.C., Mitchell D.A., Krieger N.. 2010. Process Biochemistry (1991), 45 (8) : p. 1348-1354.

DOI: 10.1016/j.procbio.2010.05.004

We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in a medium free of co-solvents, with the reaction mixture being continuously circulated through the column. The best conversion was 95% after 46 h, which was obtained at 50 ?C, with an alcohol:oil molar ratio of 3:1, alcohol addition in two steps and the addition of 1% of (m/m) water to the reaction medium. This system has potential to decrease the costs of enzyme-catalyzed transesterification reactions.

Mots-clés : pseudomonas cepacia; saccharum officinarum; helianthus annuus; glycine max; biocarburant; lipase

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